Design of CRISPR-Cas12a (Cpf1) guide RNAs

The CRISPR-Cas12a (TTTN + 23bp) System allows targeting of alternative sites that are not available to the CRISPR-Cas9 System and produces a staggered cut with a 5′ overhang. More ....

Advantages:

  • Enables genome editing in organisms with AT-rich genomes
  • Allows interrogation of additional genomic regions compared to Cas9
Please input a valid Gene Id or Genome Position or Sequence.

Please input a valid PAM DNA Sequence (Only Capital Letter).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .