Design of CRISPR-Cas12b (C2c1) guide RNAs

The CRISPR-Cas12b (TTN + 20bp) is an RNA-guided endonuclease that can specifically cleave target double stranded DNA in the presence of PAM.
Cas12b has high cleavage activity, and the optimal temperature of cleavage reaction is 48 °C, which makes it nearly impossible to use it in mammalian and plant cells. Fortunately, scientists have found several other Cas12b variants which cleave DNA at lower temperatures. More ....

Advantages:

  • Cas12b nucleases are smaller than Cas9 and Cas12a/Cpf1
  • Cas12b can maintain high enzyme activity in a wide temperature and pH range
  • Cas12b have very high target specificity (low off-target editing)
Please input a valid Gene Id or Genome Position or Sequence.

Please input a valid PAM DNA Sequence (Only Capital Letter).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .