Design of CRISPR/Cas9 guide RNAs

CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.More ....

Components:

  • Guide RNA (gRNA or sgRNA), a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20 nucleotide spacer that defines the genomic target to be modified.
  • CRISPR-associated endonuclease (Cas protein)
Please input a valid Gene Id or Genome Position or Sequence.

Please input a valid PAM DNA Sequence (Only Capital Letters with more than 2 letters).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .