Design of CRISPR activation guide RNAs

CRISPR activation (CRISPRa) is an optimized method for specific gene overexpression. CRISPRa uses an inactivated CRISPR-Cas9 system (dCas9) to upregulate target genes within their native context. This method offers many advantages over more traditional gene overexpression techniques, such as cDNA and ORF. More ....

Advantages:

  • Temporary or sustained overexpression options
  • CRISPR-based gene targeting specificity
  • Upregulation within the gene's native context
  • Natural cellular post-transcriptional processing
  • Highly biologically relevant overexpression models
Please input a valid Gene Id or Genome Position or Sequence.

When inputting the gene ID, the 2000bp sequence upstream of the gene is selected by default for sgRNA design.
Or you can modify the parameter 5' flanking sequence length (upstream) to specify the length.

Please input a valid PAM DNA Sequence (Only Capital Letters with more than 2 letters).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .

Please input a valid Upstream Length (100-5000).