Design of CRISPR Knock-ins guide RNAs

CRISPR Knock-in experiments, wherein researchers insert a gene of interest at the specific site, rely on HDR. This mechanism uses a homologous template to repair DSBs and is therefore highly accurate. The precision of the HDR repair pathway can be coupled with the specificity of CRISPR-Cas to introduce the desired sequence into the target genomic region.More ....

Optimizing Success With CRISPR Knock-in Experiments:

  • Choose the Right Guide RNA
  • Pick the Right DNA Donor Template
  • Single-stranded DNA (ssDNA)
  • Avoid Re-cutting by Cas9
  • Optimizing HDR efficiency over NHEJ
Please input a valid Gene Id or Genome Position or Sequence.

Please input a valid PAM DNA Sequence (Only Capital Letters with more than 2 letters).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .