Design of Epigenetics Editing guide RNAs

The epigenome involves a complex set of cellular processes governing genomic activity.

Epigenome Editing (EE) enables researchers to activate and repress endogenous gene expression and can provide graded control over gene regulation. Recruitment of epigenome editing effector domains using CRISPR/Cas systems allows site-specific control over modifications to DNA, histones, and chromatin architecture. More ....

Available modifications include:

  • histone acetylation
  • histone demethylation
  • cytosine methylation
  • cytosine demethylation
Please input a valid Gene Id or Genome Position or Sequence.

Please input a valid PAM DNA Sequence (Only Capital Letters with more than 2 letters).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .