Design of Fragment Deletion Editing guide RNAs

Base editors (BE) have two principal components that are fused together to form a single protein: (i) a CRISPR protein, bound to a guide RNA, and (ii) a base editing enzyme, such as a deaminase, which carries out the desired chemical modification of the target DNA base. More ....

Advantages:

1) The creation of precise, predictable and efficient genetic outcomes at a targeted sequence 2) High efficiency editing without need for template-based homology directed repair, and 3) Avoidance of the unwanted consequences of double-stranded DNA breaks.

Note: N bp (500bp is recommended) sequence on the left and right of deletion site in the direction of 5'-3'.

Please input a valid DNA Sequence.

Note: N bp (500bp is recommended) sequence on the left and right of deletion site in the direction of 5'-3'.

Please input a valid DNA Sequence.

Please input a valid PAM DNA Sequence (Only Capital Letters with more than 2 letters).
Please input a valid Spacer Length (10-50).

Note: For a Customized PAM select Customized PAM: 5'-XXX-3' in PAM Type and then set sgRNA module and Spacer length .